MAMMOTH

MAGEL2 Multi-Modal-Omics Tool Hub

Welcome to the MAMMOTH interactive data portal. This application provides tools to explore multi-omics datasets (transcriptome, proteome, and ubiquitome) from human induced pluripotent stem cell (hiPSC)-derived cortical neurons to investigate MAGEL2-associated diseases. Use the navigation panels below or the tabs at the top to access the different viewers.


Visualize the expression of individual genes.

Explore differential expression and enrichment results.

Deconvolve multi-omic variability.

Learn more about the project and data.




Overview of trained Multi-Omics Factor Analysis (MOFA) Model

Total Variance Explained


Factor Scatter Plot Matrix

Factor Correlations




About the MAMMOTH Application

General Information

The MAGEL2 Multi-Modal-Omics Tool Hub (MAMMOTH) application is an interactive, open-access web portal developed to empower the research community and facilitate the exploration of this complex dataset. It provides complete and user-friendly access to the entire transcriptome, proteome, and ubiquitinome datasets presented in the study, enabling researchers to independently visualize findings, test novel hypotheses, and accelerate progress in understanding the molecular basis of MAGEL2-related neurodevelopmental disorders.

The data originates from a comprehensive multi-omics analysis of CRISPR/Cas9-engineered isogenic human pluripotent stem cell (hiPSC)-derived cortical neurons published in the Buecking et al., 2025 preprint.

Background

Variants in the gene MAGEL2 are associated with the severe neurodevelopmental disorders Prader-Willi Syndrome (PWS) and the more severe Schaaf-Yang Syndrome (SYS), yet the underlying molecular pathophysiology in the human brain remains poorly understood. Despite known links to processes like endosomal trafficking and protein ubiquitination, the specific role of MAGEL2 in human cortical neurons during critical developmental stages has been unclear.

This project addresses this gap by presenting the first integrative multi-omics analysis for MAGEL2-related disorders.

Feedback and Contact

This application is under active development. We welcome any feedback, bug reports, or suggestions for new features. For inquiries, please contact the Laugsch Lab at Heidelberg University.

Contact: jannis.buecking@gmail.com

Lab Website: Laugsch Lab

Funding

This project was funded by the Foundation for Prader-Willi Research (FPWR).